Isolation of bacteria from goat milk with high somatic cell counts
Keywords:
infections, mastitis, Staphylococcus, antibiogramAbstract
Infections caused by bacteria are a serious problem in goat milk production, causing diseases such as mastitis, so the objective is to determine which are the main bacteria that affect the goats of the Apaseo el Grande and their relationships with the somatic cell count. The milk samples were taken in Apaseo el Grande, Guanajuato, Mexico, from a flock of 300 goats, 15 goats with signs of mastitis were selected, the samples were transported to the Veterinary Medicine and Animal Husbandry Laboratory of the University of Guanajuato, during the months of August 2011 to June 2012. For the bacteriological analysis, the California Mastitis Test, the Colony Forming Unit Count, the Catalase Test and Gram Stain, the selective media Chromagar, Baird Parker Agar and Blood Agar and antimicrobial susceptibility tests on Mueller-Hinton agar were performed. In total, 9 samples had growth and the most frequently found bacteria in the isolates was Staphylococcus aureus and, less frequently, Staphylococcus saprophyticus and Bacillus cereus. In the antibiogram, it was observed that several samples were resistant to one of the antibiotics. The main pathogen present in the milk was Staphylococcus aureus, and it is related to the somatic cell count.
References
Araya V, Gallo L, Quesada C, Chaves C, Arias ML. 2008. Evaluación bacteriológica de la leche y queso de cabra distribuidos en el área Metropolitana de San José, Costa Rica. Archivos Latinoamericanos de Nutrición. 58 (2): 182-185.
http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0004-06222008000200010&lng=es&tlng=es.
Barboza-Corona JE, De la Fuente-Salcido N, Alva-Murillo N, Ochoa-Zarzosa A. López-Meza JE. 2009. Activity of bacteriocins synthesized by Bacillus thuringiensis against Staphylococcus aureus isolates associated to bovine mastitis. Veterinary Microbiology. 138 (1–2): 179–183. https://doi.org/10.1016/j.vetmic.2009.03.018
Bergonier D, De Crémoux R, Rupp R, Lagriffoul G, Berthelot X. 2003. Mastitis of dairy small ruminants. Veterinary Research. 34 (5):689–716.
https://doi.org/10.1051/vetres:2003030
Clavijo A, Meléndez B, Clavijo M, Godoy A, Santander J. 2002. Efecto del sistema de explotación sobre la aparición de mastitis caprina en dos fincas del estado Falcón, sus agentes etiológicos y la resistencia a antimicrobianos. Zootecnia Tropical. 20(3):383-395. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0798-72692002000300008&lng=es&tlng=es
Contreras A, Corrales JC, Sanchez A, Sierra D. 1997. Persistence of Subclinical Intramammary Pathogens in Goats Throughout Lactation. J Dairy Sci. 80(11):2815–2819. https://doi.org/10.3168/jds.S0022-0302(97)76245-3
Contreras A, Paape MJ, Miller RH. 1999. Prevalence of subclinical intramammary infection caused by Staphylococcus epidermidis in a commercial dairy goat herd. Small Rumin. Res. 31 (3): 203-208. https://doi.org/10.1016/S0921-4488(98)00147-3
Contreras A, Luengo C, Sánchez A, Corrales JC. 2003. The role intramammary pathogens in dairy goats. Livestock Prod. Sci. 79 (2–3): 273-283.
https://doi.org/10.1016/S0301-6226(02)00172-0
De Buyser M, Dufor B, Maire M, Lafarge V. 2001. Implication of milk and milk products in food-borne diseases in France and in different industrialized countries. International Journal of Food Microbiology. 67 (1–2): 1-17.
https://doi.org/10.1016/S0168-1605(01)00443-3
De Cremoux R, Poutrel B, Berny F. 1995. Use of somatic cell counts for presumptive diagnosis of intramammary infections in goats. The 3rd International Mastitis Seminar, Proceedings II S6: 90.
https://agris.fao.org/search/en/providers/122439/records/64747270425ec3c088f2bc2f
Haenlein GFW. 2002. Relationship of somatic cell counts in goat milk to mastitis and productivity. Small Rumin. Res. 45(2):163–178.
https://doi.org/10.1016/S0921-4488(02)00097-4
Jayarao B, Donaldson S, Straley B, Sawant A, Hedge N, Brown JA. 2006. Survey of Foodborne Pathogens in Bulk Tank Milk and Raw Milk Consumption Among Farm Families in Pennsylvania. Journal of Dairy Science. 89(7):2451-2458.
https://doi.org/10.3168/jds.S0022-0302(06)72318-9
Kalogridou-Vassiliadou D. 1991. Mastitis-related pathogens in goat milk. Small Rumin. Res. 4 (2): 203-212. https://doi.org/10.1016/0921-4488(91)90066-Y
Leitner G, Merin U, Silanicove N. 2004. Changes in milk composition as affected by subclinical mastitis in goats. Journal of Dairy Sciece. 87(6):1719-1726.
https://doi.org/10.3168/jds.S0022-0302(04)73325-1
López MJ, Higuera RJ, Ochoa ZA, Chassin NO, Valdez AJ, Bravo PA, Baizabal AV. 2006. Caracterización molecular de aislamientos de Staphylococcus spp. asociados a mastitis bovina en Tarímbaro, Michoacán. Téc. Pecu. Méx. 44 (1): 91-106.
http://www.redalyc.org/articulo.oa?id=61344108
Norma Oficial Mexicana NOM-110-SSA1-1994. Bienes y servicios. Preparación y dilución de muestras de alimentos para su análisis microbiológico.
https://salud.gob.mx/unidades/cdi/nom/110ssa14.html
Muehlherr J, Zweifel C, Corti S, Blanco J, Stephan R. 2003. Microbiological Quality of Raw Goat´s and Ewe´s Bulk-Tank Milk in Switzerland. Journal of Dairy Sciece. 86(12): 3849- 3856. https://doi.org/10.3168/jds.S0022-0302(03)73992-7
Paape MJ, Capuco AV. 1997. Cellular Defense Mechanisms in the Udder and Lactation of Goats. J Anim Sci. 75(2):556-565. https://doi.org/10.2527/1997.752556x
Pellegrino MS, Frola ID, Odierno LM, Bogni CI. 2011. Mastitis Bovina: Resistencia a antibióticos de cepas de Staphylococcus aureus asiladas de leche. REDVET. Revista Electrónica de Veterinaria. 12 (7): 1-14.
http://www.redalyc.org/articulo.oa?id=63622567006
Petersson-Wolfe CS, Mullarky IK, Jones GM. 2010. Staphylococcus aureus Mastitis: Cause, Detection, and Control. Virginia Cooperative Extension. 404-229.
Poutrel B, De Cremoux R, Ducelliez M, Verneau D. 1997.Control of intramammary infections in goats: impact on somatic cell counts. J Anim Sci. 75 (2): 566-570.
https://doi.org/10.2527/1997.752566x
Raynal-Ljutovac K, Pirisi A, De Cremoux R, Gonzalo C. 2007. Somatic cells of goat and sheep milk: analytical, sanitary, productive and technological aspects. Small Rumin Res. 68 (1-2):126-144. https://doi.org/10.1016/j.smallrumres.2006.09.012
Salvatore V, Christian S, Francesca C, Vincenzo S, Carlo S, Enrico L. 2010. Antibiotic resistance in Staphylococcus aureus and coagulase negative Staphylococci Isolated from goats with subclinical mastitis. Veterinary Medicine International. e517060. https://doi.org/10.4061/2010/517060
Suarez MJ. Arias ML, Gamboa MM. 2008. Detección de la enterotoxina A de Staphylococcus aureus mediante la reacción en cadena de la polimerasa (PCR) y su correlación con las pruebas de coagulasa y termonucleasa. Archivos Latinoamericanos de Nutrición. 58 (1): 59-63. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0004-06222008000100008&lng=es&tlng=es
Villanueva TG, Morales CS. 2017. Resistencia antibiótica de patógenos bacterianos aislados de mastitis clínica en bovinos de crianza intensiva. REDVET. Revista Electrónica de Veterinaria. 18(12):1-12. https://www.redalyc.org/articulo.oa?id=63654640046